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Enzymes


            
             Enzymes are large molecules of amino acids that have various shapes and functions. As naturally-occurring biological catalysts, they aid in everything from digestion in humans to the decay of wood and leaves. They also represent a logical tool for pulp and paper production. The world's largest industrial use of enzymes is in starch hydrolysis and the production of syrups and prepared foods.
             Industrial and Commercial Use of Enzymes: .
             The industrial use of enzymes spans a variety of applications, such as:.
            
             • "stonewashing" blue jeans.
            
             • clarifying apple juice.
            
             • improving the quality of baked goods.
             .
             Industrial applications of these materials rely on natural enzymatic mechanisms, which are refocused and intensified to achieve desired results. Since pulp and paper is based on organic fibres, enzyme-based approaches are a natural solution.
             .
             Immobilised enzymes:.
             An immobilised enzyme is an enzyme literally that has been attached to a support that takes the form of small beads. The techniques for immobilisation vary. .
             1. Chemical (covalent) attachment of the enzyme to a supporting material. .
             2. Gel entrapment: the enzyme is mixed with gel-forming ingredients and when the gel forms the enzyme remains "trapped" in the gel matrix. The pores are large enough to let the substrate in, but not the enzyme out. .
             3. Adsorption to various surfaces is sometimes used but because the attachment is not permanent this method is usually only used for scientific studies or for "disposable" enzymes. .
             4. Encapsulated in a compartment behind a semi-permeable (porous) membrane .
             5. Crosslinking of enzyme molecules .
             The immobilised enzyme beads are added into a reaction tank and are recovered later by sedimentation or they are set up in a reactor column so that liquid flows continuously past the beads. .
             Immobilisation was actually developed in the 1950s. The first commercial use of immobilised enzymes was by the Tanaka Seiyaku company in Japan, where an immobilised aminoacylase was used to resolve amino acids into their L and D isomers.


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