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The Effect of Temperature on P

It is this characteristic that can be exploited to test which conditions affect the integrity of the cell membrane.
             Because we are experimenting with the effects of temperature on the membrane, we will place the samples of beetroot into a water baths of varying temperatures and measure the colour change in the water. Temperature is just one of the possible variables. Others include effects of poisonous substances such as alcohol and/or various solvents. The dependant variable (DV) in this experiment is colour change in water caused by anthocyanin leakage.
             Materials:.
             Beetroot Plant Corers (various diameters) White tile Heat proof mat Bunsen burner Tripod Gauze Beaker (for water bath) Thermometer Colorimeter Distilled Water7 test tubes containing 10cm³ of waterTongsScalpelStop-clockTest tube rack.
             Methods:.
             Before the experiment can start, the beetroot must first be prepared. To do this, we need the white tile and the corers. The same diameter corer must be used for all the pieces so keep the surface area of each beetroot piece fairly similar. To collect a cylinder of beetroot, simply push the corer into the vegetable and then withdraw it. The cylinder will remain inside the corer, so it must be pushed out with a corer of a smaller diameter. Once a few good, uniform cylinders have been collected, they must then be cut into 7 pieces of equal length. The beetroot was cut to 1cm. Because the beetroot has been cut some of the cell membranes had been broken, which means some anthocyanin will leak out. This must be completely washed off in order to maintain the reliability of the results.
             A water bath must then be heated to 85
             •XC (the maximum temperature for our experiment) using the Bunsen burner and tripod. Once the water bath is at the correct temperature (measured using our thermometer), one piece of beetroot is placed into the hot water directly and left for exactly 1 minute.


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