This experiment was performed to determine optimal growing conditions of Vibrio natriegens. Thirty milliliters of Vibrio broth was put into two side-armed flasks. They were both inoculated with one milliliter of Vibrio natriegens. One flask was allowed to grow in a shaker incubator and one in a regular incubator. The percent transmittance of the flasks was measured periodically using a spectrophotometer. The broth that was incubated in the aerated condition, the shaker incubator, showed more growth than the one grown under nonaerated conditions, the stationary incubator.
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INTRODUCTION.
The bacterium Vibrio natriegens is an organism that can double with a generation time of less than 10 minutes. This is important because generation times depend not only on the genetic material of a bacterial species but also on the environment in which that species is growing. (Aiyar, Gaal, Gourse, 2002) .
This experiment was conducted in order to create a growth curve for V. natriegens. A growth curve is the increase and decrease of cells versus time. To create this growth curve one must observe the pattern in which this bacteria follows. This pattern has four distinct phases: the lag phase, the logarithmic (log) phase, the stationary phase, and the death phase. From the period of inoculation to the beginning of the log phase is known as the lag phase. During this period the cells are preparing to divide. The log phase is the second phase of bacterial cell growth; during this phase the cells are doubling. The next phase is the stationary phase were the number of new cells equals the number of cells that are dying. The last phase is the death phase, in which the number of cells dying outnumbers the cells that are being made. ("Determination of a Bacterial Growth Curve").
The rate at which bacterial cells double is known as the generation time. One can determine the generation time of Vibrio natriegens by determining the turbidity ,or cloudiness, of the Vibrio broth.
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