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Catalase activity in pig mucle and liver

            
             In this Experiment The Effect of Temperature on Catalase Activity in the Liver and Muscle Tissue of Pigs, it was sought to determine if there is any correlation between temperature and catalase activity in the two different tissue types. The types of tissue used were the muscle and liver tissue of pigs because it has been shown that the anatomy of pigs is very much analogous to that of humans, and therein through a better understanding of the catalase activity in pigs there can be a better understanding of the same activity in humans. This study fits well into the realm of Metabolic Molecular Biology. The study of the catalase activity in different tissue types is important because catalase, along with super oxide dismutase, acts to break down the reactive oxygen species into water and oxygen. This metabolic breakdown is important because the reactive oxygen species has been linked to cancer. Catalase is responsible for detoxifying hydrogen peroxide and preventing the formation of carbon dioxide bubbles in the blood. Large amounts of catalase are generally found in the liver because the liver is one of the organs responsible for the breakdown of toxic material, i.e. the reactive oxygen species and hydrogen peroxide. Studies have shown skeletal muscles generate considerable reactive oxygen during intense muscle contraction. "Evidence that muscle conditioning results in upregulation of antioxidant defenses also suggests a close relationship between reactive oxygen and contractile activity" (Thomas L. Clanton). This experiment saught to determine if the aforementioned generation of reactive oxygen during intense muscle contraction is accompanied by increased catalase levels as well. In using temperature as the independent variable it was intended to recreate the heat stress on the muscle that is often associated with intense physical activity. The liver is a good comparison for the muscle tissue because it is known that with increased reactive oxygen levels catalase levels will also increase, but little is known about the effect of increased reactive oxygen levels and catalase activity in muscle tissue.


Essays Related to Catalase activity in pig mucle and liver

1. Science Lab - Understanding Enzymes

Increasing the surface area by crushing each cell type will increase the catalase activity. 3. The liver, and chicken breast animal cells will have greater catalase activity than the potato, and apple plant cells. ... Increasing the surface area by crushing each cell type will increase the catalase activity. 3. The liver, and chicken breast animal cells will have greater catalase activity than the potato, and apple plant cells. ... Lastly, in regards to the type of tissue, the following hypothesis was tested, 'the liver, and chicken breast animal cells will have a greater catalase activi...

  • Word Count: 1941
  • Approx Pages: 8
  • Has Bibliography
  • Grade Level: High School

2. Lab - Effects of pH on the Activity of Catalase

Extremely high or low pH values generally result in complete loss of activity for most enzymes. ... Any temperature apart from the optimum is unsuitable to work in as it can affect the rate of reaction and we are not looking at how temperature affects the activity but we are looking at how pH affects the activity. ... Maintaining the volume of catalase or enzyme you use is very important as the more/less available active sites are present which overall affects the activity of the catalase as the substrate which is hydrogen peroxide will have less/more to catalyse. Concentration of Catalase ...

  • Word Count: 2380
  • Approx Pages: 10
  • Grade Level: High School

3. Catalase

MATERIALS AND METHODS: PART A: In the fist part of the lab, the catalase is mixed with substrate (hydrogen peroxide). ... Before all of the catalase is converted, sulfuric acid (H2SO4) stops the reaction. ... PART C: To determine the decomposition rate or H2O2 after using a purified catalase extract, place 10 mL of H2O2 and 1mL of catalase in a beaker and swirl for 10 seconds before adding 10 mL of H2SO4 to stop the reaction. ... DATA: Catalase Activity KMnO4(mL) 10 seconds 30 seconds 60 seconds 120 seconds 180 seconds Baseline 3.3 3.3 3.3 3.3 3.3 Final reading 8.5 11.2 13.7 25 3.7 Init...

  • Word Count: 854
  • Approx Pages: 3
  • Grade Level: High School

4. School

Hypothesis: The non-protein catalyst (potato) will be less active when compared to the catalase in the liver. ... When the used piece of liver was added to the fresh H2O2 the reaction preceded exactly the same as the original reaction with the fresh liver. ... I assume that a dog liver contains the same catalase as the cow liver that we used; therefore, the results would be the same. 5. We used liver because it contains the protein enzyme, catalase in large amounts. Conclusions With both the catalase, and non-protein catalyst: - An increase in heat decreased the rate of reaction...

  • Word Count: 366
  • Approx Pages: 1
  • Grade Level: High School

5. Decomposition of hydrogen peroxide.

At 0oC one molecule of catalase can decompose 40,000 molecules of Hydrogen Peroxide a second. ... When the pH is altered above or below this the rate of the enzyme activity diminishes. ... Catalase is found in the liver and in certain plant cells such as the cells in a potato. I will be using liver as a source of catalase during this experiment. ... For each test the 1g piece of liver is to be as a whole chunk, to make the surface area of the liver equal for each test, this will make the results more accurate. ...

  • Word Count: 2761
  • Approx Pages: 11
  • Grade Level: High School

6. To investigate the factors that affects the reactivity of the enzyme Catalase.

Catalase is found in almost any kin of living cells. ... Enzymes are also affected by temperature, this increases their activity. ... Factors, which affect the activity of an enzyme; Temperature; Temperature affects the activity of an enzyme by doubling the activity with a increase of 100C. ... The amount of Catalase. ... This is because there will be more Catalase to react with. ...

  • Word Count: 565
  • Approx Pages: 2
  • Grade Level: High School

7. Temperature and Substrate Concentration on Enzyme Activity

The main findings of this experiment lead to the discovery that optimal temperature of catalase is around 35°C-50°C with a slight anomaly of a fast activity rate at 10°C. ... The dependent variables for the experiment are, the enzyme activity, the type of substrate being used (in this lab it was H2O2 ) and the type of catalase. ... A dropper was then used to drop 3 drops of 1% catalase onto 3 different catalase disks. ... The activity of the enzyme catalase decreases at higher temperatures because enzymes are proteins, and proteins are denatured at high temperatures. ... Because the activi...

  • Word Count: 2220
  • Approx Pages: 9
  • Has Bibliography

8. A Temperature Effect

(http://www.chem.wsu.edu 2) In this experiment the purpose was to see if temperature has an effect on the activity of catalysts. METHODS & MATERIALS (Gunstream, 1999) Celsius thermometer Crushed Ice Dropper Hydrogen peroxide Liver extract Metric ruler Test-tubes Test-tube racks Water Starting the experiment, a drop of the liver extract and a dropper of tap water were placed into four tubes. ... However when the liver extract was tested at 85 C there was no catalyst activity. The colder the temperature, the lower the catalyst activity. ... Among the temperatures tested, boiling...

  • Word Count: 510
  • Approx Pages: 2
  • Has Bibliography
  • Grade Level: High School

9. Liver

In 2002 more than 17,000 patients were in need of a liver transplant. ... The main problem and obstacle of developing a way to replace the functions of liver is duplicating the many important functions the liver is responsible for. ... On a brighter note there have been many breakthroughs in replicating the livers functions. There have been some support systems developed to mimic some of the functions of the liver. ... The first of this type of this system was the HemoTherapies Liver Dialysis unit. ...

  • Word Count: 460
  • Approx Pages: 2

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