For each test ran in each separate lab with each separate reagent, there is a known positive and negative result. We want to see which substances will test negative or positive for containing Sugar or Fat Molecules. .
Hypothesis .
Sugar – If we test DI Water for sugar I didn't expect it to test positive. I was correct. H2O doesn't naturally contain any sugars. If we test Starch for sugar I did expect it to test positive for sugar but I was incorrect. I expected this because starches and sugars are in the same food group on the food pyramid, I was always taught they were both unhealthy interchangeable molecules. If we test any type of sucrose level, either one or ten percent I did expect it to test positive for sugar and I was correct. I believed that sucrose was a liquefied version of sugar molecules. If we tested onion juice for sugar content, I didn't believe it would test positive for sugar because there is nothing sweet about an onion. Surprisingly I was incorrect and the onion juice actually contained the highest level of sugar content. .
Fats.
If we place a drop of water on a brown bag, my educated guess was that the water will evaporate and test negative for fat content. I was correct. If I place a drop of vegetable oil or evaporated milk I didn't expect it to fully evaporate without leaving an oily residue, because oil will always leave oil behind and because milk can't fully evaporate at room temperature. .
Materials & Methods .
A Test Tube Rack, 4 to 5 Test Tubes, Transfer Pipet, Protective Glasses, DI Water (deionized water), 1% Sucrose Solution, 1% Starch Solution, Onion Juice, 10 % Sucrose Solution, Benedicts Reagent, Evaporated Milk, Boiling Water, Beaker, Test Tube Holder for safety, Brown Paper Bag Squares (3).
Methods .
Even though the lab protocol for performing the test for proteins, starches and sugars in a substance is relatively similar, there are different methods used to test for certain molecules and their content levels.
