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Decomposition of H2O2

            An Experiment to investigate how temperature affects the rate of reaction between Catalase and hydrogen peroxide.
             Aim.
             This experiment is designed to see how temperature affects the breakdown of hydrogen peroxide by the enzyme catalase. .
             Scientific Knowledge.
             Enzymes are biological catalysts. They are produced in all living organisms and control all the chemical reactions that occur. Most of the chemical reactions that occur in living organisms would occur too slowly without enzymes. Increased temperatures would speed up reactions but using enzymes means that the reactions are fast enough at body temperature. .
             Enzymes are protein molecules that have a particular shape. They have a slot or a groove into which the substrate fits. The reaction then takes place and the products leave the enzyme. .
             This explains why enzymes are specific. Each enzyme is designed to fit only one substrate. .
             Catalase is a very fast reacting enzyme; it is found in many living cells, it breaks down hydrogen peroxide to water and oxygen. One molecule of it can break down 6million molecules of hydrogen peroxide in 1 minute. Hydrogen peroxide needs to be broken down into harmless substances to protect the body from it as it is toxic. Hydrogen peroxide is a problem for all living cells as it is a by product of a number of chemical reactions that take place in each cell. Catalase is present in nearly all aerobic cells. It serves to protect the cell from the toxic effects of hydrogen peroxide. This is done by enabling the decomposition of hydrogen peroxide into its molecular oxygen and water constituents without, the production of free radicals. The effects of the experiment are illustrated below:.
             Hydrogen peroxide - water + oxygen.
             2H2O (aq) - 2H2O (l) + O2 (g).
             If the decomposition process free radicals, i.e. an atom or group of atoms with an unpaired electron, were produced this may be harmful to the cell. This is because radicals are unusually reactive and are capable of causing a wide range of biological damage.


Essays Related to Decomposition of H2O2

1. Catalase

The enzyme catalyzes the conversion of H2O2 to H2O and O2. ... PART C: To determine the decomposition rate or H2O2 after using a purified catalase extract, place 10 mL of H2O2 and 1mL of catalase in a beaker and swirl for 10 seconds before adding 10 mL of H2SO4 to stop the reaction. ... HYPOTHESIS: If enzyme catalase is included in the assay solution the rate at which the H2O2 decomposes to H20 and oxygen will increase. ... This number is proportional to the amount of H2O2 in the solution. ... This decrease corresponds with the decomposition of the H2O2 solution into its respective parts ...

  • Word Count: 854
  • Approx Pages: 3
  • Grade Level: High School

2. Catalase Enzyme Detection

Materials: 1 clean microscopic slide, 3% H2O2 solution, swabs. ... In addition of H2O2 Patient G cells also show positive reaction forming oxygen and water which means it decomposes hydrogen peroxide into water and oxygen. ... Enterococcus faecalis doesn't show produce bubbles in addition of H2O2 which means it doesn't produce oxygen and is catalase negative. Conclusion/Interpretation: Bubbles appeared after placing H2O2 shows positive reaction to the catalase enzyme. ... The primary reaction catalyzed by catalase is the decomposition of hydrogen peroxide to form water and oxygen, ...

  • Word Count: 686
  • Approx Pages: 3
  • Grade Level: High School

3. Decomposition of hydrogen peroxide.

Decomposition of hydrogen peroxide. Planning- Introduction: In this experiment I will be testing the rate of reaction of the decomposition of Hydrogen Peroxide using the enzyme Catalase when an inhibitor is present. ... Catalase: Catalase is an enzyme which catalyses the decomposition of Hydrogen Peroxide, as shown in the equation; Catalase 2H2O2 2H2O + O2 ...

  • Word Count: 2761
  • Approx Pages: 11
  • Grade Level: High School

4. Experiment 11

You will consider the following reaction types: combination reactions, decomposition reactions, single-replacement, double-replacement reactions, and combustion reactions. ... Data Record Table 11.1 Observations Reaction Observations Reaction Type Fe and CuSO4 Pb(NO3)2 and Kcl Mg and HCl H2O2 and heat electrolysis of H2O NaHCO3 and heat Data Analysis 1. ...

  • Word Count: 759
  • Approx Pages: 3
  • Grade Level: High School

5. Rates of Reaction Coursework - Hydrogen Peroxide and Catalas

This is because there will be less Hydrogen Peroxide in the beaker so there will be less to decompose. ... As explained in My Investigation', its scientific formula is H2O2. ... Such factors as light, heat, chemical catalysts, dirt, and rust may cause hydrogen peroxide to decompose into water, oxygen, and heat. ... To keep hydrogen peroxide from decomposing, it is kept in dark bottles and at low temperatures. ... Hydrogen peroxide acts as both an oxidizing and reducing agent, because the oxygen freed in decomposition readily combines with other substances. ...

  • Word Count: 2748
  • Approx Pages: 11
  • Grade Level: High School

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