1. NIH 3T3 Cells - Confocal Microsopy
In this experiment, using confocal microscopy, two organelles were visualized to understand the drug effect of nocodazole on NIH3T3 cells. ... After red medium was extracted from cell, paraformaldehyde fixative was added and removed multiple times to ensure cells were killed off but still maintained cellular structure and conformational shape. ... Glass slides were kept at room temperature for minimal time before given to lab instructor for confocal microscope visualization (Katula). ... In untreated cells with Hoechst 33342 stain, blue structure showed organelle was concentrated to visualize...
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- Approx Pages: 2
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- Grade Level: Undergraduate