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Human Blood

(This served only as a reference point).
             6) The coarse focus was then slowly turned anti clockwise whilst looking down the eyepiece lenses until the specimen came into view.
             7) The condenser was focussed on the specimen. This was done by closing the iris on the field lens (by twisting the outside of the field lens). When looking down the eyepiece lenses a black circle with a pale, blurred centre was observed (d. 1.1). .
             d. 1.1.
             The condenser control was then adjusted until the pale centre became a crisp hexagonal shape (d.1.2).
             d. 1.2.
             8) The condenser iris was adjusted. (This regulated how much light illuminated the image). Firstly, one eyepiece lens was removed and the condenser iris was closed using the black switch on the side of the iris. The image observed whilst looking down the eyepiece opening was a dark circle with a small speck of light at the centre (d. 2.2).
             d. 2.1.
             The condenser iris was then adjusted using the aforementioned switch until approximately three quarters of the field of view was filled with light (d.2.2).
             d. 2.2.
             9) The eyepiece lens was then inserted again. The microscope was now ready to use.
             10) Observations were made of the ileum.
             11) The low power objective lens (x 10) was replaced with a high power objective .
             (X 40) by revolving the nosepiece.
             12) As the lenses are par-focal, the image was further focussed until the features were sharp using the fine focus.
             13) Step 8 was repeated.
             14) Further observations were made of the ileum.
             15) The high power objective lens (x 40) was replaced with the x 63 lens, which further magnified the image.
             16) Steps 12-13 were repeated.
             17) Steps 2-16 were repeated using a blood smear stained with Leishmann's stain.
             Results.
             .
             The erythrocytes were the most plentiful of the cells observed. They had no nucleus and the centre of the cells were stained paler than the perimeter. The erythrocytes were smaller than the leucocytes observed but several times larger than the platelets.


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