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Electrophoresis

 

An electric current flowing across the covering carries the various fractions horizontally as they descend, depositing each in a separate collecting tube at the bottom edge. .
             Electrophoresis is used in many different areas of science. This technology is demonstrated in the following areas: Electrophoresis of Protein, Electrophoresis of DNA, Gel Electrophoresis, and Polyacrylamide Electrophoresis. These methods of electrophoresis are unique but all lead to the goal of obtaining the specific identity, measurement, and separation of the substance being tested.
             Protein electrophoresis is used to evaluate, diagnose and monitor a variety of diseases and conditions. It can be used for these purposes because the levels of different blood proteins rise and fall in response to such disorders as cancer, intestinal or kidney protein-wasting syndromes, disorders of the immune system liver dysfunction impaired nutrition, and chronic fluid-retaining conditions.
             Proteins are major components of muscle, enzymes, hormones, hemoglobin, and other body tissues. A protein is any of the group of highly complex organic compounds found in all living cells and comprising the most abundant class of all molecules. An enzyme is a catalyst ( used to speed up chemical reactions). A hormone is a substance carried from one gland or organ of the body by the bloodstream to tissue, where it exerts influence upon the metabolism of the target tissues. Proteins are composed of elements that can be separated from one another by several different techniques: chemical methods, centrifuge (devise used to separate two or more substances of different density), or electrophoresis. There are two major types of electrophoresis: protein electrophoresis and immunoelectrophoresis. Immunoelectrophoresis is used to assess the blood levels of specific types of proteins called immunoglobulins. Protein electrophoresis is used to determine the total amount of protein in the blood, and to establish the levels of other types of proteins called albumin, alpha1 globulin, alpha2 globulin, and beta globulin.


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