Investigation to see the rate of oxygen produced by the enzyme catalase when combined with H202.
Aims: To investigate how the reaction between the enzyme catalase and H202 is effected by the concentration of catalase. .
Catalysts accelerate chemical reactions that otherwise proceed slowly. The enzyme called.
catalase is a catalyst. It exists in plant and animal cells and breaks down hydrogen peroxide,.
H2O2, which is a byproduct of metabolism. Hydrogen peroxide is toxic if it accumulates in a.
cell.
The chemical reaction accelerated by catalase is written.
2H2O2 > 2H2O + O2 (gas) .
The products of the above reaction are oxygen gas and water, two non-poisonous molecules. In living cells, the oxygen can be used for cellular respiration and the water can be excreted.
Apparatus:.
Test Tube.
Bung.
Measuring cylinder.
Tray.
Goggles.
Knife.
Potato.
H202 (20 vol).
Potato cutting surface.
Access to distilled water.
Your apparatus must be set out thusly:.
Under favorable conditions, the reaction occurs very fast. The maximum catalytic rate for one.
catalase molecule is 6 million molecules of hydrogen peroxide converted to water and oxygen.
per minute. The reaction product is 6 million molecules of water and 3 million molecules of.
oxygen. .
Variables.
The concentration of H2O2 .
The concentration of catalase.
If the solution is heated.
If the solution is cooled.
Control of variables.
It is important that only the concentration of the enzyme catalase changes. All other variables must be kept the same (controlled variables) since if they are changed it will effect the reaction rate.
Brief outline of how enzymes work.
An enzyme is a protein molecule that speeds up chemical reactions. Each enzyme has a unique shape that determines its function. One enzyme works on one type of substrate. .
The enzyme, Amylase has a shape that allows its to wrap around starch(substrate) and cut it up into individual glucose units.
Predictions.
If i change the concentration of H2O2 i predict that more oxygen will be produced at a faster rate i say this because there will be more H2O2 for the enzyme to react with than if the concentration were less.