Cellular respiration

             In the first part of the lab study we investigated the effects of yeast concentration on the rate of alcoholic fermentation.We hypothesized that the Fermentation rate will be greater in the fermentation tube with more yeast. And our prediction was that if fermentation is greater in the fermentation tube with more yeast, then more CO2 Will be produced in the tube. In table 1.1, test tube 1 was used as a control with no yeast, while test tube 2 also as a control with no glucose. Test tube 3 and 4 are the experimental tubes. Tube 4 has 3 times as much yeast as tubes 2 and 3. .
             Test tube 4 had the highest rate of fermentation because it contained both the substrate (glucose) and the highest amount of yeast (containing the enzymes). Whereas there was no fermentation in test tube 1 because there was no yeast. Test tube 2 with yeast but no substrate didn't have fermentation. And different amounts of water were added to each fermentation solution to maintain the appropriate Concentrations of yeast in each tube.Since tube 4 had the highest rate of fermentation supports the hypothesis because tube 4 had the highest concentration of the substrate (glucose).
             In the second part of the lab study we investigate the effect that changing the amount of substrate (succinate) will have on the cellular respiration rate. We hypothesized that as the substrate (succinate) increases the rate of cellular respiration will increase. And our prediction was that if increased substrate increases the rate of cellular respiration, then respiration will be greater in the solution with more Succinate and this tube should turn from blue to clear more rapidly.
             The experimental mixtures like mitochondrial suspension, Succinate, DPIP, bufffer have specific roles. The mitochondrial suspension provides the enzymes, succinate acts as a substrate, DPIP acts as an electron acceptor, and the buffer maintains the optimum PH for the reactions.