And Shoots on Nicotine production in tobacco.
The article "Effect of Regenerated Roots and Shoots on Nicotine Production I tobacco Tissue Culture of J. Saunders fully follows the IMRD structure. The article, as it appears in the Drug Information Journal (32(1998):609), is divided into four general sections. The general sections are: introduction, materials and methods, results and discussions, and references. The purpose of this paper is to resolve what relationship the different organs of the tobacco plant might have in the production of nicotine. From this information, the author concludes that there is an active turnover mechanism operating in shoots of tobacco culture, which requires a prodigious synthesis of nicotine in the plant to maintain high levels of nicotine found in tobacco issue. .
The introduction of this article is consistent with the goals settled by the IMRD Introduction section. In the first paragraph, the author defines the use of tissue culture that their research focuses on and the facts that influences the variability of chemical constitution from intact plants. It also states the reason of the research that give rise to their research like finding out the reason in the differences of the level of chemical constitution of the plants. This is just one of the consequences of their purpose of the research. The second and third paragraph of the introduction section gives the historical background of the study. It tells us truly that there are differences in the use of tissue culture for production system for plant components. The last paragraph clearly stated the purpose of the paper: to know what takes place in the organs of the plants on the accumulation of alkaloids and its mobilization. .
The Materials and Methods section are clearly categorized into four subsections: Plant Material, Growth Conditions of Callus Cultures, Extraction and Quantitation of Nicotine by High Pressure Liquid Chromatography (HPLC), and Chemicals.