Cell Adhesion and Extracellular Components

It is converted to fibrin when blood clots. This investigation looks at the ability of three extracellular components: type I collagen, Laminin-1 and fibrinogen to adhere to C6 glioma cells. .
             Results.
             Before trypsin was added the cells were all clustered together and also stuck to the plate wall. After trypsinisation the cells were separated from each other and also the plates. After fixation the cells in matrix 1 and 2 were seen to be in clusters similar to before trypsinisation and the cells in matrix 3 did not change much after the addition of trypsin, i.e. they were separated from each other. .
             Calculation of number of cells plated per well:.
             Average number of cells (A) = 67.
             Number of cells in cell suspension = 67 x 104 per ml.
             Therefore, in 100 µl = 67 x 104 10 = 67 x 103 per ml = 6.7 x 104 cells per ml.
             Table 1: Mean, SD and SE of Optical Density at 630nm of C6 glioma cells in different matrix components.
             Optical Density.
             Optical Density.
             Optical Density.
             Optical Density.
             Mean.
             Standard Deviation.
             Standard Error.
             Matrix 1.
             1.118.
             1.106.
             1.059.
             1.062.
             1.09.
             03.
             02.
             Matrix 2.
             1.161.
             1.134.
             1.148.
             1.136.
             1.14.
             01.
             01.
             Matrix 3.
             754.
             1.107.
             799.
             774.
             86.
             17.
             08.
             BSA.
             597.
             751.
             618.
             508.
             62.
             10.
             05.
             Med.
             948.
             888.
             966.
             966.
             94.
             04.
             02.
             Serum.
             1.141.
             1.027.
             1.228.
             1.112.
             1.13.
             08.
             04.
             Optical Density.
             Optical Density.
             Optical Density.
             Optical Density.
             Mean.
             Standard Deviation.
             Standard Error.
             Matrix 1.
             272.
             241.
             117.
             21.
             84.
             07.
             03.
             Matrix 2.
             225.
             197.
             16.
             194.
             776.
             03.
             01.
             Matrix 3 .
             048.
             033.
             053.
             045.
             179.
             01.
             00.
             Discussion.
             From the observation and the results it can be seen that matrix 1 and 2 adhered to the C6 cells more than matrix 3. The addition of trypsin re-suspended the cells from the walls and from each other. When the different matrix types were added the components of the matrix bound to their respective integrin and hence got re clustered except in the case of matrix 3. When the cells were fixed all cellular movements ceased hence enabling us to identify the most and the least adherence ability. Optical density is the negative log of transmittance. The optical density is directly proportional to the level of adherence.