(Vo) Reaction time (min) Product concentration (µM) (using [S]=0,05 mM) Product concentration (µM) (using [S]=0,5 mM) 0 0 0 2,5 8,77 29,2 5 19,7 58,4 10 34,8 117 20 40 132 40 42,5 143 Table 1 In experiment 2, we determined the optimal temperature for AP, alkaline phosphatase. ... For example Blank: B 0oC, Sample: S 0oC, B 20oC, S 20oC and so on. ... In this graph below we see the linear progression of our product formation curves during the first 10 min of the reaction, until the linear line started to deviate. ... Graph 1. ... Graph 2. ...

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Results: The corresponding graphs can be found on following page(s). Fig. 1: The graph shows an increase in the reaction rate as the enzyme concentration increases. ... Fig. 2: The graph shows the increase of the substrate concentration generates an increase in the rate of the reaction. ... Fig. 3: The graph shows the absorbance increasing briefly then decreasing as the temperature increases. ... s 1 and 2). ...

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The rate of reaction was .14 (cm/s) for the Catalase that was 90 degrees Celsius and .23 (cm/s) for the Catalase that was 50 degrees Celsius. ... Also looking at Graph number three it can be seen that the optimal temperature for an enzyme to be efficient out of the tested temperatures is, 30 degrees Celsius. ... This surprising outcome was an outlier on the graph and set of information we acquired through the lab. ...

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The small disk s of filter paper were made before hand using a hole punch. ... Discussion Based on Figure 1's results, enzyme activity decreases at higher temperatures and increases at lower temperatures. ... Based on the trend shown from the parabola in the graph, the optimum temperature range is from 35-50°C, but can also include 10°C as well as seen in the results of this lab. ... This can be seen in Figure 2 because the graph curves downwards as the substrate concentration increases. ...

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