1. Research Proposal - Proteins
The sequences of the stalk proteins suggest that they are either inner-membrane proteins (CC0498 and CC1953) or of unknown location (CC3022) (12). However, none of these proteins have a known signal sequence for secretion. ... Using an in vivo pulse-chase type of experiment, I will test whether stalk proteins are delivered directly to the stalk. ... A range of xylose concentrations will be tested for protein induction to find the minimal concentration needed for visible expression of the Eos-fusion. ... To test this possibility, I have identified a protein, CC1398, which when fused to mCh...
- Word Count: 4190
- Approx Pages: 17
- Grade Level: Undergraduate